Fig. 2

Sample growth and curving during the live imaging experiment. Stereoscope images at 50 × magnification of leaf samples with no coverslip and also no cotyledon dissection A, C or samples with dissection and affixed coverslip B, D for the same* WT (A–B, white) and jaw-D (C–D, yellow) samples over the course of a live imaging experiment. Dissected samples affixed with a cover slip maintain positions with more exposed leaf tissue for imaging over time. Both WT and jaw-D leaves begin to grow out from the plate without coverslip dissection exposing the adaxial side. This is especially true in jaw-D where the tissue becomes curled around itself at 7 DAS. *5 DAS image was missing for this sample so an image from a different WT leaf sample is provided. Arrows indicate leaf that was imaged. Yellow scale bars = 2 mm