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Fig. 3 | Plant Methods

Fig. 3

From: The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning

Fig. 3

3D-models and fully assembled Rapid-Tome. A The handle and stage are connected with a single metal bolt through holes “A” after sliding the slider piece into the vertical grooves from the top. B The assembly of the sled includes the attachment of the blade clamp with the blade clamp nut, blade clamp washer, and blade clamp screw. The blade clamp nut should be slid into place first. The blade clamp screw is fed through the top of the blade clamp with the blade clamp washer between. The blade guard should be kept in place over the blade at all times when the sled is removed from the stage. The blade guard is removed during sectioning and stored in the holster contained within the sled. C The fully assembled Rapid-Tome with advancement bolt, stage washer, and blade installed

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