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Fig. 6 | Plant Methods

Fig. 6

From: Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis

Fig. 6

Protein–protein interaction assays in cotton root protoplasts. A BiFC analysis of the interaction between GhBIN2 and GhBZR3 was conducted in cotton root protoplasts. The paired constructs p326-GhBIN2-YFPN (GhBIN2-YFPN) and p326-GhBZR3-YFPC (GhBZR3-YFPC) were transiently co-expressed in protoplasts. GhBIN2-YFPN or GhBZR3-YFPC with the empty vector (p326-YFPC or p326-YFPN) were used as negative controls. Merge, merged images of YFP and bright field. Bars = 10 μm

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Plant Methods

ISSN: 1746-4811