Fig. 4From: Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysisPCR/RE assays to detect CRISPR/Cas9-induced mutations in cotton protoplasts. a and b The activities of two sgRNAs were detected. Lane 1: digested PCR products amplified from samples treated with the respective sgRNAs. Lane 2: the digested wild-type control. Lane 3: the undigested wild-type control. Red arrowheads indicate bands with mutations. The sgRNA target sequence is in blue; the protospacer adjacent motif is in greenBack to article page