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Fig. 4 | Plant Methods

Fig. 4

From: Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis

Fig. 4

PCR/RE assays to detect CRISPR/Cas9-induced mutations in cotton protoplasts. a and b The activities of two sgRNAs were detected. Lane 1: digested PCR products amplified from samples treated with the respective sgRNAs. Lane 2: the digested wild-type control. Lane 3: the undigested wild-type control. Red arrowheads indicate bands with mutations. The sgRNA target sequence is in blue; the protospacer adjacent motif is in green

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