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Fig. 2 | Plant Methods

Fig. 2

From: Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis

Fig. 2

Optimization of the protoplast isolation protocol for single-cell sequencing. a Effects of using different mannitol concentrations in the resuspending solution on protoplast activity (0.3, 0.4, 0.5, 0.6, and 0.7 M). b The proportion of LSCs (≥ 40 μm) observed at different mannitol concentrations. c and d Compared with using a 40 μm cell strainer (c), LSCs were significantly reduced in number by using 40 and 30 μm cell strainers, successively (d). Red arrows mark LSCs. Bars = 100 μm. e Effect of different filtration methods on the proportion of LSCs in the cell suspension. f Expression analysis of genes involved in epigenetic modification between digested protoplasts and unprocessed root tissue

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Plant Methods

ISSN: 1746-4811