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Fig. 6 | Plant Methods

Fig. 6

From: Identification of an emerging cucumber virus in Taiwan using Oxford nanopore sequencing technology

Fig. 6

Detection of CBLV infection in field cucumber crops by indirect ELISA (a) and RT-PCR (b). The produced RAs-CBLV was used in indirect ELISA. The primers CBLV3900F and CBLV4576R were used in RT-PCR amplification. Cucumber samples, ‘2106-1’ to ‘2106-5’, collected from Xizhou, Changhua in June 2020 were used for CBLV detection. Sample ‘2106-2’ is the source of CBLV-TW. CBLV-TW-infected and healthy Chenopodium quinoa leaf tissues were used as positive and negative controls, respectively. The expected size of amplicons in RT-PCR assay is indicated by an arrow

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