Fig. 4
From: Identification of an emerging cucumber virus in Taiwan using Oxford nanopore sequencing technology
Purification of CBLV-TW coat protein (CP) from infected Chenopodium quinoa leaves by ultra-speed centrifugation method. a An obvious opalescent band indicated by a white arrow was observed after isopycnic centrifugation through 32% cesium sulfate. b Individual centrifugation fractions of purification procedures, 10 K rpm, 25 K rpm and 45 K rpm, were analyzed by 12% SDS-PAGE. S and P represent supernatant and pellet, respectively. c Immunoblotting using the produced antiserum RAs-CBLV was conducted to detect the purified CBLV CP (lane CP). The crude extract of CBLV-TW-infected C. quinoa leaf was used as positive control (lane CBLV). Protein marker (lane M) was loaded as molecular weight standard. CBLV-TW CP is indicated by black arrows