Table 1 Effect of different enzyme concentration combinations on mesophyll protoplast isolation in C. oleifera
Treatment No. | Enzyme solution combination(%) | Protoplast yield( × 106/g·FW) | Protoplast viability(%) | ||||
|---|---|---|---|---|---|---|---|
Cellulase R-10 | Macerozyme R-10 | Pectolyase Y-23 | Hemicellulase | Snailase | |||
1 | 1.0 | 1.0 | 0 | 0 | 0 | 0.07 ± 0.003d | 61.7 ± 5.32d |
2 | 1.0 | 1.0 | 0.25 | 0 | 0 | 0.61 ± 0.064 cd | 83.1 ± 1.40abc |
3 | 1.5 | 0.5 | 0.25 | 0 | 0 | 0.78 ± 0.045 cd | 76.6 ± 1.38c |
4 | 1.5 | 1.0 | 0.05 | 0 | 0 | 0.77 ± 0.048 cd | 81.5 ± 3.35abc |
5 | 1.5 | 0.5 | 0 | 0.5 | 0 | 0.08 ± 0.003d | 62.4 ± 5.04d |
6 | 1.5 | 0.5 | 0 | 0 | 0.2 | 20 ± 4.92b | 89.65 ± 1.27a |
7 | 1.5 | 0.5 | 0 | 0 | 0.25 | 35 ± 4.05a | 90.9 ± 2.18a |
8 | 1.0 | 1.0 | 0 | 0 | 0.25 | 19 ± 3.15b | 87.2 ± 1.43ab |
9 | 1.5 | 1.25 | 0 | 0 | 0.2 | 7.7 ± 0.512c | 80.1 ± 1.16abc |
10 | 1.5 | 1.25 | 0 | 0 | 0.25 | 6.2 ± 0.698 cd | 79.7 ± 1.33bc |