Fig. 2

Effects of mannitol concentration, pretreatment method and duration of enzyme application on mesophyll protoplasts isolation in C. oleifera. A Effects of mannitol concentration in enzyme solution (enzyme composition in 1.0% Cellulase R-10 and 1.0% Macerozyme R-10) on protoplast isolation; B effects of vacuum treatment on mesophyll protoplasts isolation in C. oleifera (Vacuum treatment 1 –5 indicates that the vacuum treatment time is 0, 10, 20, 30 and 60 min respectively); C effects of dark treatment on mesophyll protoplasts isolation in C. oleifera (Dark treatment 1–6 indicates that the dark treatment time is 0, 12, 24, 30, 36 and 40 h, respectively); D effects of duration in enzyme application (enzyme composition in 1.5% Cellulase R-10, 0.5% Macerozyme R-10 and 0.25% Snailase) on protoplast isolation. Different letters represent a statistically significant difference at P < 0.05, and bars represent standard errors