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Fig. 5 | Plant Methods

Fig. 5

From: A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis

Fig. 5

The comparison of intron splicing status in different CB-RNAs. A The cartoons show different fractions of the chromatin-bound RNAs. RNAe indicates the nascent being transcribed at the locus. RNAf indicates the full-length RNA, which is polyadenylated yet still attached to the chromatin. B Boxplot showed splicing ratio of 5ʹ SS and 3ʹ SS ratio of RNAe, RNAf, and RNAe + RNAf. ***p < 0.001, Wilcoxon test. The boxplot showed the median and the 25th and 75th percentiles. C The relationship between co-transcriptional splicing (CTS) and intron order in RNAe, RNAf, and RNAe + RNAf. The genes containing 9 introns were selected as representatives. X-axis indicated the intron order from 1st to 9th. The analyzed gene number was indicated at the top of each box. D The relationship between co-transcriptional splicing (CTS) and intron number in RNAe, RNAf, and RNAe + RNAf. The genes containing 1 to 10 introns were selected as representatives. X-axis indicated the intron number from 1 to 10. The analyzed gene number was indicated at the top of each boxplot

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