Fig. 1
From: A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis
A brief workflow of chromatin-bound RNA extraction in plants. A The cartoon demonstrates the workflow of chromatin-bound RNA extraction in Arabidopsis. B Western-blot detection of different proteins in different fractions that are obtained by chromatin-bound RNA extraction. Note that the UBC1 is absent from the nucleoplasm and chromatin fraction, while H3 and Pol II are highly enriched in the chromatin fraction. C Gel-electrophoresis detection of RNAs in different fractions. Note that 28S and 18S rRNA bands can be observed clearly in different fractions, indicating RNA degradation is minimal during extraction