Fig. 3

IMPLANT results in T0 of O. sativa. A. Overview of the modified hygromycin resistance marker in pICSL11059, with indication of the competitor (341 bp) and the amplified fragment of the endogenous O. sativa sequence (377 bp). B. IMPLANT results using capillary gel electrophoresis. The signal intensity of the competitor is set relative to the intensity of the endogenous sequence (dotted green line). The correction factor was empirically determined and set at 0.65. Corr., corrected; Inferr., inferred. C. ddPCR analysis results. Bars show the distribution of the number of molecules of the competitor (orange) and the endogenous sequence (green). The dotted lines indicate the theoretical distribution corresponding to a given copy number