Fig. 5From: A low-cost and open-source solution to automate imaging and analysis of cyst nematode infection assays for Arabidopsis thalianaThe spectrum of coloured agar under various lighting conditions. A The range of colours tested for contrast with nematodes and filtration potential of the background. B The colour red agar under four different lighting conditions. From left to right: White, Red, Green and Blue light. The blue lighting condition with the red agar were the most ideal combination for quantifying nematodes using the segmentation tool. The bottom image row has the contrast and brightness adjusted for visibility. Examples of female nematodes are highlighted with yellow arrowsBack to article page