Fig. 1From: X-ray fluorescence spectroscopy (XRF) for metallome analysis of herbarium specimensThe effects of thickness on the XRF readings. Red lines are a model fitted into the reported concentrations (black dots) following Eq. (1). The samples (black dots) are constant in concentration but vary in thickness. The model reaches a plateau at a certain thickness that signifies the escape depth or critical thickness of the respective elements and shifts towards the right direction of the x-axis as the atomic number of the elements gets higher. Small variations in the sulphur and potassium XRF results are due to random noise and indicate that the samples are considered as thick samples for XRF. The underlying data used is provided in Additional file 1Back to article page