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Fig. 3 | Plant Methods

Fig. 3

From: A robust and efficient automatic method to segment maize FASGA stained stem cross section images to accurately quantify histological profile

Fig. 3

Result of the proposed workflow on F4 and F7 maize inbred lines cross section FASGA stained images. A F4 inbred line FASGA stained internode cross sections images. B F7 inbred line FASGA stained internode cross sections images. C F4 segmented images resulting from the proposed workflow. D F7 segmented images resulting from the proposed workflow. E Quantified area of lignified parenchyma tissues MT1a and MT4a (on top and bottom, respectively) from F4 (in blue) and F7 (in pink) cross section, expressed in percentage of medullary tissues (%MT). F Quantified area of lignified parenchyma tissues MT1b and MT4b (on top and bottom, respectively) from F4 (in blue) and F7 (in pink) cross section, expressed in percentage of medullary tissues (%MT). G Quantified area of low lignified parenchyma tissues MT2a and MT5a (on top and bottom, respectively) from F4 (in blue) and F7 (in pink) cross section, expressed in percentage of medullary tissues (%MT). H Quantified area low lignified parenchyma tissues MT2b and MT5b (on top and bottom, respectively) from F4 (in blue) and F7 (in pink) cross section, expressed in percentage of medullary tissues (%MT). I Magnifications of bundles from F4 inbred line FASGA stained cross section images obtained in panel A. J Segmented images of F4 inbred lines bundles. K Magnification of bundles from F7 inbred line FASGA stained cross section images obtained in panel B. L Segmented images of F7 inbred line bundles. M Quantified area of bundle tissues (BT1, BT4, BT2 and BT5) from F4 (in blue) and F7 (in pink) cross section, expressed in percentage of bundle tissues (%BT). N Magnification of rind region of F4 inbred lines FASGA stained cross section image. O Magnification of rind region of F7 inbred lines FASGA stained cross section image. P F4 ET1 segmented images tissues from the region presented in panel N. Q F7 ET1 segmented tissues from the region presented in panel O. R F4 DRT8 and DRT9 segmented tissues from region magnified in panel N. S F7 DRT8 and DRT9 segmented tissues from region magnified in panel O. T Quantified sclerenchyma hypodermis tissues ET1 from F4 (in blue) and F7 (in pink) cross section, expressed in percentage of rind tissues (%RT). U Quantified area of sclerenchyma tissues DRT8 and DRT9 (on the left and on the right, respectively) from F4 (in blue) and F7 (in pink) cross section, expressed in percentage of rind tissues (%RT). Significance: no symbol, p ≤ 0.1; *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. A, B, C and D Scale bar = 1 cm. I, J, K and L Scale bar = 100 µm. N, O, P, Q, R and S Scale bar = 500 µm

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