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Fig. 5 | Plant Methods

Fig. 5

From: Combining BN-PAGE and microscopy techniques to investigate pigment-protein complexes and plastid transitions in citrus fruit

Fig. 5

Blue-native PAGE profile and immunoblot with antibodies against photosynthesis-related proteins from isolated plastids at green, breaker and orange color stages. A Blue-native PAGE profile of chloroplast membrane protein complexes at green stage. Molecular mass markers are indicated on the left of the figure. B Separation of membrane protein complexes from chloroplasts (left lane), chlo-chromoplasts (middle lane) and chromoplasts (right lane) by BN-gel electrophoresis. Red asterisks indicate the bands present in all three periods. Each lane of BN-gel was loaded with 200 μg of protein. Immunoblotting analysis showing changes in the relative amounts of protein subunits in PS I complexes (C) and PS II complexes (D). Each lane of immunoblotting analysis was loaded with 20 μg of protein

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Plant Methods

ISSN: 1746-4811