Table 1 LC–MS/MS method parameters
From: High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing
Analyte | Abbreviation | Multiple reaction monitoring transitionsa | Collision energy, eVa | Ionisation | Retention time, min b | Internal standard | Limit of detection, fmol c | Linear range, pmol | pKa d |
|---|---|---|---|---|---|---|---|---|---|
indole-3-acetic acid | IAA | 176.1–130.1 | 24 | [M + H]+ | 6.85 ± 0.01 | [13C6]-IAA | 0.5 | 0.005-50 | 4.66 |
2-oxindole-3-acetic acid | oxIAA | 192.1–146.0 | 12 | [M + H]+ | 4.34 ± 0.01 | [13C6]-oxIAA | 10 | 0.05–50 | 3.74 |
indole-3-acetyl aspartate | IAA-Asp | 291.1–130.1 | 36 | [M + H]+ | 5.54 ± 0.01 | [13C6]-IAA-Asp | 25 | 0.05–50 | 3.74 |
indole-3-acetyl glutamate | IAA-Glu | 305.2–130.1 | 24 | [M + H]+ | 5.94 ± 0.01 | [13C6]-IAA-Glu | 2.5 | 0.005—50 | 3.65 |
indole-3-acetyl-1-O-ß-D-glucose | IAA-glc | 336.1–174.0 | 8 | [M - H]− | 4.02 ± 0.02 | [13C6]-IAA-glc | 10 | 0.05–50 | 5.10 |
2-oxindole-3-acetyl-1-O-ß-D-glucose | oxIAA-glc | 352.2–190.0 | 8 | [M - H]− | 2.66 ± 0.02 | [13C6]-oxIAA-glc | 1 | 0.005–50 | NP |
cis-( +)-12-oxo-phytodienoic acid | cis-OPDA | 293.2–275.2 | 12 | [M + H]+ | 13.80 ± 0.01 | [2H5]-OPDA | 2.5 | 0.005–5 | 4.78 |
(Z)-8-[3-oxo-2-(pent-2-enyl)cyclopentyl]octanoic acid | OPC-8 | 295.2–135.0 | 20 | [M + H]+ | 14.36 ± 0.01 | [2H5]-OPDA | 25 | 0.05–5 | 4.72 |
(Z)-6-[3-oxo-2-(pent-2-enyl)cyclopentyl]hexanoic acid | OPC-6 | 267.1 – 135.0 | 28 | [M + H]+ | 13.18 ± 0.01 | [2H5]-OPDA | 25 | 0.05–5 | 4.65 |
(Z)-4-[3-oxo-2-(pent-2-enyl)cyclopentyl]butanoic acid | OPC-4 | 237.2 – 58.8 | 20 | [M - H]− | 11.71 ± 0.01 | [2H6]-JA | 25 | 0.05–50 | 4.55 |
dinor-oxo-phytodienoic acid | dn-OPDA | 265.2 – 247.1 | 4 | [M + H]+ | 12.51 ± 0.01 | [2H2]-JA-Ile | 25 | 0.05–5 | 4.60 |
(−)-jasmonic acid | JA | 209.2–58.8 | 8 | [M - H]− | 10.08 ± 0.01 | [2H6]-JA | 0.75 | 0.005–50 | 4.71 |
(±)-methyl jasmonate | MeJA | 225.3 – 151.2 | 12 | [M + H]+ | 11.71 ± 0.01 | [2H6]-MeJA | 2.5 | 0.005–5 | NP |
(±)-9,10-dihydrojasmonic acid | 9,10-dhJA | 211.2–58.8 | 16 | [M - H]− | 11.09 ± 0.01 | [2H6]-JA | 2.5 | 0.005-50 | 4.77 |
11-hydroxyjasmonic acid/ 12-hydroxyjasmonic acid | 11-/12-OHJA | 225.1–59.0 | 8 | [M - H]− | 5.13 ± 0.03 | [2H6]-JA | 5 | 0.05–50 | 4.46 |
(−)-jasmonyl-L-valine | JA-Val | 310.3–151.3 | 16 | [M + H]+ | 11.60 ± 0.01 | [2H2]-JA-Ile | 1 | 0.005–5 | 4.01 |
(−)-jasmonyl-L-isoleucine | JA-Ile | 324.3 – 151.2 | 16 | [M + H]+ | 12.22 ± 0.01 | [2H2]-JA-Ile | 2.5 | 0.005–5 | 4.06 |
(−)-jasmonyl-L-tryptophan | JA-Trp | 397.3–351.3 | 12 | [M + H]+ | 12.00 ± 0.01 | [2H2]-JA-Ile | 50 | 0.1–5 | 3.39 |
(−)-jasmonyl-L-phenylalanine | JA-Phe | 358.8–151.2 | 16 | [M + H]+ | 12.52 ± 0.01 | [2H2]-JA-Ile | 2.5 | 0.005–4.5 | 3.97 |
salicylic acid | SA | 137.1–92.8 | 16 | [M − H]− | 8.03 ± 0.03 | [2H4]-SA | 25 | 0.05–50 | 2.79 |
(+)-cis,trans-abscisic acid | ABA | 263.2–153.1 | 8 | [M − H]− | 9.10 ± 0.01 | [2H6]-ABA | 0.25 | 0.005–50 | 4.50 |
phaseic acid | PA | 279.1–205.1 | 12 | [M − H]− | 6.61 ± 0.01 | [2H3]-PA | 5 | 0.01–50 | 4.30 |
dihydrophaseic acid | DPA | 281.2–237.1 | 8 | [M − H]− | 4.96 ± 0.02 | [2H3]-DPA | 25 | 0.05–50 | 4.34 |
neophaseic acid | neoPA | 279.1–205.1 | 12 | [M − H]− | 8.41 ± 0.00 | [2H3]-neoPA | 1 | 0.005–50 | 4.30 |
7'-hydroxyabscisic acid | 7'-OHABA | 279.1–151.1 | 12 | [M − H]− | 7.90 ± 0.01 | [2H4]-7'-OHABA | 5 | 0.01–50 | 4.37 |