Fig. 7
From: Photolysis of caged cytokinin in single cells of Arabidopsis thaliana
Monitoring cell wall damage following irradiation with a 720 nm MP confocal laser (70% intensity). Cells of A. thaliana roots before (A, C, E, G), and immediately after (B, D, F, H) MP irradiation. White arrows/dotted lines highlight bulges or punctures in the cell walls. Red or yellow boxes indicate region of irradiation. G and H Propidium iodide (3 mg/ml), applied to the roots ten minutes before irradiation, highlighting cell wall structures, shown in magenta. Images A–F taken using transmitted light; images G and H captured using 488 nm reflected fluorescence optics