Fig. 3

Fluorescence stereomicrographs displaying GFP expression of TCSn::GFP roots following uncaging treatments. 7-day old seedlings were treated with CA or CBA, as well as solutions of these molecules that had been previously uncaged by UV illumination for one hour (as well as positive and negative control treatments). The top row shows the roots at time 0. A Solvent control (0.5% DMSO); B 100 µM CA; C 100 µM uncaged CA; D 100 µM CBA; E 100 µM uncaged CBA; F 0.1 µM BA. Scale bar: 500 µm. Quantification of this fluorescence is shown in Additional file 1: Fig. S12