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Fig. 2 | Plant Methods

Fig. 2

From: A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean

Fig. 2

Mapping and modifying regions of CPSMV RNA1 cDNA conferring bacterial toxicity. A Mapping regions in RNA1 cDNA conferring toxicity to E. coli. Diagrams of five deletion mutants are presented, with the deleted region denoted with dashed lines. The one-column table to the right summarizes the effect of the deletions on E. coli growth rate, which correlates inversely with the toxicity of RNA1 cDNA. B Positions of CPSMV RNA1 cDNA into which various introns have been inserted in order to improve the replication efficiency of the corresponding constructs. All intron-containing constructs are listed in the table to the right, along with the intron(s) they possess, and their impacts on E. coli and A. tumefaciens growth rates. hr hours, WT wildtype, NT not tested

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