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Fig. 1 | Plant Methods

Fig. 1

From: A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean

Fig. 1

CPSMV genome organization and a two-step strategy for assembling a full-length cDNA clone of CPSMV RNA1. A Diagrams of CPSMV RNA1 and 2, the encoded ORFs, and the nt coordinates of various mature proteins. Both genomic RNAs are depicted as thick lines. The small gray oval at the left end denotes the VPg covalently linked to the RNAs, whereas An at the right end denotes a poly-A tail. The numbers above each line denote the nt positions that border various viral proteins, which are in turn depicted as boxes of different colors. B Strategy of cloning RNA1 cDNA into pAIDE. The 2X35S promoter and T35S transcriptional terminator in pAIDE ensure the efficient and proper transcription of the inserted RNA1 cDNA. The first cloning step integrates two short terminal fragments, shown as black and light gray boxes respectively, that form a new SmaI site at their junction. The second step then integrates the intervening fragment(s) of the cDNA into the SmaI site

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