Skip to main content
Fig. 1 | Plant Methods

Fig. 1

From: A simple and efficient method to quantify the cell parameters of the seed coat, embryo and silique wall in rapeseed

Fig. 1

Flowchart of the procedure for quantifying seed coat cell parameters. a Seed images are acquired under a stereomicroscope. b The seed images are processed with Fiji software to calculate the seed area. c The seed coats are peeled off and photographed under an optical microscope. d The cell images are processed using the TWS–Fiji-based method (TF method) or the Cellpose–MorphoLibJ–LabelsToROIs-based method (CML method) to obtain the average cell size. Left, TF method; right, CML method; TWS, Trainable WEKA Segmentation. e The number of cells is calculated as the seed surface area divided by the average cell size

Back to article page

Plant Methods

ISSN: 1746-4811