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Fig. 6 | Plant Methods

Fig. 6

From: Combination of long-term 13CO2 labeling and isotopolog profiling allows turnover analysis of photosynthetic pigments in Arabidopsis leaves

Fig. 6

Mass spectra of Lut extracted from non-labeled and 13C-labeled Arabidopsis plants. FTICR-MS showing four different types of quasi-molecular ions of Lut, [M + H–2H2O]+, [M + H–H2O]+, [M]+ and [M + H]+, in a non-labeled (a) and a 13C-labeled (c) sample. Small peaks of 13C-labeled [M + H–2H2O]+ ion were detected in the same m/z region as non-labelled [M]+ and [M + H]+ ions in c. Deviations from the expected mass (Δ) are given in ppm. TQ-MS showing four types of quasi-molecular ions, [M + H–2H2O]+, [M + H–H2O]+, [M]+ and [M + H]+, in the same non-labeled (b) and 13C-labeled (d) samples as in a and c. Since TQ-MS cannot separate overlapping peaks of non-labeled [M]+ and [M + H]+ at m/z 569–571 and 13C-labeled [M]+ and [M + H]+ at m/z 607–608, they are regarded as [M + H]+. Peak assignment of these data is summarized in Additional file 2: Tables S7–S10

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