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Fig. 4 | Plant Methods

Fig. 4

From: Combination of long-term 13CO2 labeling and isotopolog profiling allows turnover analysis of photosynthetic pigments in Arabidopsis leaves

Fig. 4

Chromatograms of a 13C-labeled Arabidopsis leaf pigment sample obtained by LC-TQ-MS. a Pigment separation monitored at 440 nm. The pigment peaks are numbered as follows: 1, Vio; 2, 9-cis-Neo; 3, Anthera; 4, Chl b; 5, Lut; 6, Zea (if present); 7, Chl a; 8, all-trans-β-Car; 9, 9-cis-β-Car. This sample had a very small amount of Anthera and hardly any Zea. In the same sample, selected ions were monitored at specific mass-to-charge (m/z) values: b Vio and 9-cis-Neo; c Anthera; d Chl b; e Lut (and Zea if present); f Chl a; g all-trans- and 9-cis-β-Car. Xanthophylls, especially Lut, tend to lose water upon protonation in positive ion mode

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Plant Methods

ISSN: 1746-4811