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Table 3 Increased number of breeding generations per year facilitated by regulated growth conditions and cultivation of plants from seeds of oven-dried immature pods

From: Cowpea speed breeding using regulated growth chamber conditions and seeds of oven-dried immature pods potentially accommodates eight generations per year

Genotype

Seed source

Growth chamber type

Days to 50% flowering ± SD (n = 3)

Pod age (DAP) at harvest

Duration of pod oven drying (days (oC))

Days from sowing to the next generation

Potential No. of cycles/year

Parents (preliminary trial)

 Sasaque

Metal halide (−)

LED (+)

32 ± 1.5

29

N/A

61

6.0

 Sasaque

Metal halide (−)

LED (−)

32 ± 1.5

29

N/A

61

6.0

 IT86D-1010

Metal halide (−)

LED (+)

40 ± 1.0

32

N/A

72

5.1

 IT86D-1010

Metal halide (−)

LED (−)

40 ± 1.0

32

N/A

72

5.1

 IT97K-499-35

Field

LED (+)

44 ± 1.0

31

N/A

75

4.9

 IT97K-499-35

Field

LED (−)

44 ± 1.0

31

N/A

75

4.9

Parents (main trial)

 Sasaque

LED (+)

LED (+)

32 ± 1.5

14

4 (25)

50

7.3

 Sasaque

LED (−)

LED (−)

32 ± 1.5

14

4 (25)

50

7.3

 IT86D-1010

LED (+)

LED (+)

40 ± 1.0

14

4 (25)

58

6.3

 IT86D-1010

LED (−)

LED (−)

40 ± 1.0

14

4 (25)

58

6.3

 IT97K-499-35

LED (+)

LED (+)

44 ± 1.0

14

4 (25)

62

5.9

 IT97K-499-35

LED (−)

LED (−)

44 ± 1.0

14

4 (25)

62

5.9

F1 generation

 Sasaque

LED (−)

Metal halide A

32 ± 1.5

11

2 (39)

45

8.1

 IT86D-1010

LED (−)

Metal halide A

40 ± 1.0

11

2 (39)

53

6.8

 IT97K-499-35

LED (−)

Metal halide A

44 ± 1.0

11

2 (39)

57

6.4

 Sasaque × IT86D-1010

LED (−)

Metal halide A

35 ± 1.5

11

2 (39)

48

7.6

 Sasaque × IT97K-499-35

LED (−)

Metal halide A

35 ± 1.7

11

2 (39)

48

7.6

 IT97K-499-35 × IT86D-1010

LED (−)

Metal halide A

42 ± 2.1

11

2 (39)

55

6.6

F2 generation

 Sasaque

Metal halide A

Metal halide A

32 ± 1.5

11

2 (39)

45

8.1

 IT86D-1010

Metal halide A

Metal halide A

40 ± 1.0

11

2 (39)

53

6.8

 IT97K-499-35

Metal halide A

Metal halide A

44 ± 1.0

11

2 (39)

57

6.4

 Sasaque × IT86D-1010

Metal halide A

Metal halide A

35 ± 1.5

11

2 (39)

48

7.6

 Sasaque × IT97K-499-35

Metal halide A

Metal halide A

35 ± 1.7

11

2 (39)

48

7.6

 IT97K-499-35 × IT86D-1010

Metal halide A

Metal halide A

42 ± 2.1

11

2 (39)

55

6.6

  1. To determine average days to flowering, each generation of the main study was considered a replicate for the parents, whereas the F1 and F2 plants were cultivated in three replicates, four plants in each replicate (n = 3)
  2. DAP, days after pollination; LED, light-emitting diodes; (−), no CO2 supplementation; (+), CO2 supplemented; N/A, not applicable; SD, standard deviation