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Fig. 5 | Plant Methods

Fig. 5

From: An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster

Fig. 5

Visualization of the de novo sequences in the PsMYB10.1a promoter region containing a G-box motif (underlined) associated with the red skin color, SNPs and two large InDels. The de novo sequences had resolution to the SNP level when compared between them and to the Sanger sequences provided in Fiol et al. Heterozygous positions (shadowed bases) on the de novo sequences matched the nucleotide on the same haplotype in a different variety (red rectangles). Sequencing mistakes are marked with asterisks

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Plant Methods

ISSN: 1746-4811