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Fig. 3 | Plant Methods

Fig. 3

From: An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster

Fig. 3

a MYB10 amplicon bands before and after cleavage with a pool of the guide RNAs (gRNAs) targeting the genes, with their size annotated in base pairs (bp). Bands (a, b) correspond to the faint bands from the allele with a large intron 1, (c) to the remaining undigested product. Wells M1: Lambda DNA HindIII-EcoRI digested molecular weight marker; M2: DNA Ladder 50 bp ready-to-use (GeneON). b Expected fragment sizes after MYB10.1 and MYB10.2 digestion with the Cas9-Ribonucleoprotein (RNP) complex. Guide-RNAs assembled with the trans-activating CRISPR RNA (tracrRNA) and crRNA-s (s1, s2 or s3) cut the exon 2 of the gene, gRNAs with crRNA-a (a1, a2, a3 or a4) cut by intron 1. Fragment 6 was not obtained after cleavage, fragment 3 and 5 bands overlapped in the gel

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Plant Methods

ISSN: 1746-4811