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Fig. 2 | Plant Methods

Fig. 2

From: An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster

Fig. 2

Schematic representation of the CRISPR RNA (crRNA) design to specifically cleave and sequence a hypothetic LG3-MYB10 genomic region in Japanese plum. The guide RNAs (gRNAs) are formed by the interaction of trans-activating RNAs with crRNAs, then with the Cas9 enzyme are assembled into the Cas9- Ribonucleoprotein (Cas9-RNP) complex. The Cas9-RNPs allows the specific cleavage of the LG3-MYB10 region, generating cuts at the two DNA strands for further sequencing in both directions from the inside to the outside of the genes. Gene number, order and size of the region, as well as the expected number of fragments were unknown at the time of the crRNA design

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Plant Methods

ISSN: 1746-4811