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Fig. 1 | Plant Methods

Fig. 1

From: Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa

Fig. 1

Effect of BrrWUSa on shoot regeneration frequency. Hypocotyl segments co-cultured with 35S:GFP (a) or 35S:BrrWUSa-GFP (b). c, d Callus formation on selection medium with kanamycin and hormone after 2 weeks in vitro culture. e, f Representative transformation showing a higher frequency of transgenic BrrWUSa plants and the empty vector controls. g Transgenic seedling on root induction medium. h Transgenic-specific PCR product amplified with primers GFP-F and BrrWUSa-R. M, DNA ladder marker DL2502. i Green fluorescence observation in the root tips of 35S:BrrWUSa-GFP transgenic plants. Scale bars = 1 mm in af, 1 cm in g and 30 μm in i

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