Fig. 6

Reproducibility and required UMI counts for targeted and non-targeted RNA-Seq. a Plot of Pearson’s correlation coefficients of the target log2 RPM + 1 between each of the subsampled 96 technical replicates and the full targeted RNA-Seq dataset. The replicates were prepared using a single tube of purified RNA from A. thaliana seedlings. b Plots of examples of the target log2 RPM + 1 in each size of subsampled sets from 12 pseudo-technical replicates of targeted and non-targeted RNA-Seq results. The same purified RNA was used for the preparation of 96 technical replicates of non-targeted RNA-Seq. Then, groups of 8 RNA-Seq results were merged, resulting in 12 pseudo-technical replicates. c Plot of Pearson’s correlation coefficients of the target log2 RPM + 1 between each subsampled set from the 12 pseudo-technical replicates and the full dataset of targeted and non-targeted RNA-Seq. a–c Red indicates targeted RNA-Seq, gray indicates non-targeted RNA-Seq, horizontal axis labels indicate the numbers of subsampled UMI counts