Fig. 5
From: DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate
Required amount of input RNA for targeted RNA-Seq. a–c The result of targeted RNA-Seq using 0.01 ng (n = 96), 0.1 ng (n = 96), 1 ng (n = 10), and 10 ng (n = 5) of purified A. thaliana RNA. A subsample of 10,000 reads from each library was used for the analyses. Boxplots of the ratio of target reads per total read (a), the numbers of detected target genes (b) and Pearson’s correlation coefficients for the results of each input amount compared to that of 10 ng RNA (c). Each point indicates the value of each technical replicate