Fig. 3
From: DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate
Direct-lysate reverse transcription generates cDNA comparable to that from reverse transcription of purified RNA. The results of 3′ RNA-Seq of purified RNA and lysate of A. thaliana seedlings. A total of 100,000 subsampled reads for each technical replicate were used for the following analyses. a Boxplot of the mapped read ratio to 100,000 reads. b Boxplot of the rRNA read ratio to 100,000 reads. c Boxplot of the number of detected genes. Each point indicates the value of each technical replicate. d Scatter plot of log2 RPM + 1 of each gene. The average of technical replicates was plotted. Differentially quantified genes (DQGs) were detected between RNA-Seq for purified RNA and lysate (FDR = 0.05). e Transcript length distribution of DQGs with higher rpm values in the method using lysate and other genes. Histograms from 0 to 6000 nt are shown