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Fig. 1 | Plant Methods

Fig. 1

From: DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate

Fig. 1

Reducing reagents inhibited RNA degradation in plant and animal tissue lysate. Bioanalyzer electropherograms of purified RNA from tissue lysates (A. thaliana seedlings, O. sativa leaves, O. sativa root, T. aestivum coleoptile, S. cerevisiae, and D. rerio) containing the reducing reagents dithiothreitol (DTT) and 2-mercaptoethanol (2ME). The columns indicate the concentrations of the reducing reagents in the homogenization buffers. N.C. represents no reducing reagents in the buffers (negative control)

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Plant Methods

ISSN: 1746-4811