Table 5 Plasmodiophora brassicae isolate mixtures generated to assess the capacity of the SNaPshot assay to determine relative abundances
From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters
Isolatesa | Proportions | Genomic DNA (ng)b |
|---|---|---|
SS48(3H): ST20(5X) | 10: 90 | 1: 9 |
20: 80 | 2: 8 | |
30: 70 | 3: 7 | |
40: 60 | 4: 6 | |
50: 50 | 5: 5 | |
60: 40 | 6: 4 | |
70: 30 | 7: 3 | |
80: 20 | 8: 2 | |
90: 10 | 9: 1 | |
F3-14(3A): LG-1(5X) | 10: 90 | 1: 9 |
20: 80 | 2: 8 | |
30: 70 | 3: 7 | |
40: 60 | 4: 6 | |
50: 50 | 5: 5 | |
60: 40 | 6: 4 | |
70: 30 | 7: 3 | |
80: 20 | 8: 2 | |
90: 10 | 9: 1 | |
SACAN-ss1(3H): LG-2(5X) | 10: 90 | 1: 9 |
20: 80 | 2: 8 | |
30: 70 | 3: 7 | |
40: 60 | 4: 6 | |
50: 50 | 5: 5 | |
60: 40 | 6: 4 | |
70: 30 | 7: 3 | |
80: 20 | 8: 2 | |
90: 10 | 9: 1 |