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Table 4 Identification of blinded root galls infected by Plasmodiophora brassicae used in the single-blind study

From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

Sample #

Isolate identification

Puritya

Pathotypeb

1

SACAN-ss3c

SSI

2F

2

ORCA-ss4

SSI

5I

3

AbotJE-ss1c

SSI

6 M

4

CDCN-ss3

SSI

8 N

5

ST27d

SSI

3H

6

ST40d

SSI

3A

7

LG-2c

FI

5X

8

F3-14c

FI

3A

9

F1-14

FI

3D

10

CDCN # 4-14

FI

3H

11

SW46d

SSI

6B

12

F185-14c

FI

3A

13

SS23d

SSI

4A

14

LG-3c

FI

5X

15

SACAN-03-1

FI

3H

16

SC14d

SSI

6A

  1. While samples had been previously pathotyped, the experiment was conducted without knowledge of pathotype designations until assays were complete
  2. aSamples were either field isolates (FI) or had been previously purified as single-spore isolates (SSI)[2, 19]
  3. bPathotype designations are based on the Canadian Clubroot Differential set[2, 4]
  4. cIsolates used in earlier testing of DNA extracted from pathotyped galls (Table 3)
  5. dSSIs used during the optimization stage of the rhPCR and SNaPshot assays (Table 2)