Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

Table 2 Single-spore isolates of Plasmodiophora brassicae used during rhPCR assay optimization

Reference 5X cluster isolates	SC14 (6A); SR20 (6B); SS23 (4A); SS25 (6B); ST11 (5X); ST16 (5X); ST20 (5X); ST23 (5X); ST25 (6B); ST26 (6B); ST29 (6B); ST49 (6B); SW46 (6B)
Alternate 3H cluster isolates	S05 (3D); S16 (2A); S36 (2F); S39 (2F); SA13 (6C); SC07 (2F); SC19 (3H); SC26 (2F); SC50 (3H); SL02 (3H); SL09 (2F); SL36 (3D); SN45 (7A); SR04 (3H); SR42 (3H); SS02 (2A); SS06 (3D); SS11 (2A); SS34 (3D); SS48 (3H); ST27 (3H); ST37 (3H); ST40 (3A); SW09 (3D); SW30 (3H);

Each single-spore isolate obtained is given a molecular identification for its isolate name. Canadian Clubroot Differential pathotype designations [2, 4] are indicated in parentheses after each isolate name

ISSN: 1746-4811