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Fig. 8 | Plant Methods

Fig. 8

From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

Fig. 8

Results of the single-blind study with the SNaPshot assay displayed with capillary electrophoresis. The SNaPshot primer snpsht1-43,778 was used to cluster the 16 blinded galls. The graphs highlight the discriminatory SNP, either an allele of A for the 5X cluster or an allele of G for the 3H cluster. Allele A fluoresces green, whereas allele G fluoresces blue. Samples were disclosed to be isolates SACAN-ss3, ORCA-ss4, AbotJE-ss1, CDCN-ss3, ST27, ST40, LG-2, F3-14, F1-14, CDCN #4-14, SW46, F185-14, SS23, LG-3, SACAN-03-1, and SC14. The sequencing results were visualized with Peak Scanner v1.0 (Applied Biosystems, Waltham, MA, USA). The size of the SNaPshot product is measured along the x-axis against the GeneScan 120 LIZ size standards (Applied Biosystems, Waltham, MA, USA)

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