Fig. 6

The capacity of the SNaPshot assay to determine the relative abundance of mixtures of Plasmodiophora brassicae isolates displayed by capillary electrophoresis. The results shown were obtained with the SNaPshot primer snpsht1-43,778. Three two-isolate mixtures were evaluated at ratios of 10:90, 20:80, 30:70, 40:60, 50:50, 60:40, 70:30, 80:20, and 90:10. The graphs highlight the relative proportion of each allele, an allele of G for the 3H cluster or an allele of A for the 5X cluster. Allele G fluoresces blue, whereas allele A fluoresces green. a The first mixture included single-spore isolates SS48 and ST20 from our original collection; b the second mixture included the field isolates F3-14 and LG-1 extracted from root galls; and c the third mixture included the single-spore isolate SACAN-ss1 and the field isolate LG-2. Sequencing results were visualized with Peak Scanner v1.0 (Applied Biosystems, Waltham, MA, USA). The strength of the fluorescence peak is measured against the vertical axis. The size of the SNaPshot product is measured along the x-axis against the GeneScan 120 LIZ size standards (Applied Biosystems, Waltham, MA, USA)