Skip to main content
Fig. 5 | Plant Methods

Fig. 5

From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

Fig. 5

The differentiating capacity of the SNaPshot assay displayed by capillary electrophoresis of DNA extracted from isolates of P. brassicae from pathotyped galls. The SNaPshot primer snpsht1-43,778 was designed against a discriminatory SNP containing an allele of A for the 5X pathotype cluster and an allele of G for the 3H pathotype cluster. Allele A fluoresces green, whereas allele G fluoresces blue. Sequencing results were visualized with Peak Scanner v1.0 (Applied Biosystems, Waltham, MA, USA). The strength of the fluorescence peak is measured against the vertical axis. The size of the SNaPshot product is measured along the x-axis against the GeneScan 120 LIZ size standards (Applied Biosystems, Waltham, MA, USA)

Back to article page