Fig. 3
From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters
Testing of the specificity of the rhPCR assay against DNA extracted from clubroot galls resulting from infection by Plasmodiophora brassicae isolates representing different pathotypes. Amplification products were resolved by electrophoresis on 1% (w/v) agarose gels prepared with Tris-acetate-EDTA buffer and SYBR Safe gel stain. A GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific, Waltham, MA, USA) was included in the leftmost lane as the marker. The primer pairs rh1-43812R (upper panel) and rh1-43812A (bottom panel) were evaluated against P. brassicae isolates SACAN-ss3, F3-14, F185-14, F189-14, SACAN-ss1, ORCA-ss3, LG-1, LG-2, LG-3, AbotJE-ss1, F187-14, and CDCN-ss1 (lanes 1–12). The corresponding pathotype of each isolate is indicated above the respective amplicon. The negative control is displayed in the rightmost lane, as represented by the N