Fig. 2
From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters
Testing of the specificity of the rhPCR assay against single-spore isolates of Plasmodiophora brassicae. Amplification products were resolved by electrophoresis on 1% (w/v) agarose gels prepared with Tris-acetate-EDTA buffer and SYBR Safe gel stain. A GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific, Waltham, MA, USA) was included in the leftmost lane as the marker. The primer pairs rh1-43812R (upper panels) and rh1-43812A (bottom panels) were evaluated against single-spore isolates; a S05, S16, S36, S39, SA13, SC07, SC14, SC19, SC26, SC50, SL02, SL09, SL36; b SN45, SR04, SR20, SR42, SS02, SS06, SS11, SS23, SS25, SS34, SS48; c ST11, ST16, ST20, ST23, ST25, ST26, ST27, ST29, ST37, ST40, ST49, SW09, SW30, and SW46. The corresponding pathotype designation of each isolate is indicated above the respective amplicon. The negative control is displayed in the rightmost lane, as represented by the N