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Fig. 3 | Plant Methods

Fig. 3

From: Generation of the salicylic acid deficient Arabidopsis via a synthetic salicylic acid hydroxylase expression cassette

Fig. 3

Growth and leaf senescence phenotypes of the S5Hpro::EGFP-S3H transgenic plants. a Morphological phenotypes of WT, NahG and S5Hpro::EGFP-S3H transgenic plants at 28 and 35 DAG, Bar = 2 cm. b Quantification of the S3H expression in WT, NahG and S5Hpro::EGFP-S3H transgenic plants at 21 DAG by qPCR. The data are means ± SE (n = 3 biological replications). c Quantification of the rosette leaf diameters from plant of (a) at 28 DAG. The data are presented as means ± SE (n ≥ 10 biological replications). d Quantification of chlorophyll content in the 5–6th leaves from plant of (a) at 35 DAG, the data are means ± SE (n = 4 biological replicates); FW, fresh weight. e Fv/Fm of the 5–6th leaves from plant (a) at 35 DAG, the data are means ± SE (n = 4 biological replicates). e Phenotypes of WT, NahG and S5Hpro::EGFP-S3H transgenic plants grown on 1/2 MS medium with or without 100 μM sodium salicylate. Bar = 1 cm. f Quantification of chlorophyll content from plant of (e), the data are means ± SE (n = 4 biological replications); FW, fresh weight. Statistical differences among replicates are labeled with different letters (P < 0.05, one-way ANOVA and post-hoc Tukey’s test)

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