Fig. 4

Proof-of-principle tethering of an RNA decay enzyme function to the SOC1 mRNA. A Translational fusion between FLAG-BD and the CAF1a deadenylase protein generates the BD + D protein. B Flowering time of BD + D plants two (T2) and three (T3) generations after transformation. + T plants inherited the BD + D transgene, -T plants are siblings that did not inherit the transgene. Box plots and statistics are the same as in Fig. 2A. C qRT-PCR of SOC1 polyadenylated mRNA. Three biological replicates of each genotype are shown as red points. Bar height, error bars and statistics are the same as Fig. 2B. D qRT-PCR of SOC1 nascent transcripts (unspliced and not polyadenylated). Three or more biological replicates of each genotype are shown as red points. Bar height, error bars and statistics are the same as Fig. 2B. E ePAT assay to determine the poly(A) tail length of the SOC1 mRNA. n = the number of clones Sanger sequenced. TVN is a control where the reverse transcription primer is anchored at the most 3’ nucleotide before the poly(A) tail begins. Box plot organization is the same as Fig. 2A. P-value is calculated by using an unpaired t-test with Welch’s correction. F Quantification of SOC1 protein accumulation in the BD + D line. Individual biological replicates are down as blue points. Bar height, error bars and statistics are the same as Fig. 2B