Fig. 2

FLAG-BD binding does not alter SOC1 regulation. A Flowering time is measured as the number of leaves generated at the time the first flower opens. Gray points are individual plants, and the red box plots represent the 25th and 75th percentiles of the sample population, with the center bar representing the median and whiskers at the 10th and 90th percentile. P-values are comparisons to the wt Col in the same growth replicate, calculated by using unpaired t-test. ns = not statistically significant. B qRT-PCR of SOC1 mRNA levels in plants with and without FLAG-BD. Three or more biological replicates for each genotype were used (shown as red points), the height of the bar represents their average and the error bars represent the standard deviation. P-value is calculated by using unpaired t-test with Welch’s correction. C Western blot displaying SOC1 protein levels between biological replicates with and without FLAG-BD. D SOC1 protein quantification from the Western blot in part (C). Biological replicate data points are shown as blue points, the height of the bar represents their average and error bars represent the standard deviation. P-value is calculated by using unpaired t-test with Welch’s correction. E Accumulation of siRNAs from wt Col and FLAG-BD lines. TAS3 is a trans-acting siRNA producing locus shown as a positive control for siRNA accumulation. AT2G20610 is an unrelated gene without small RNA production used as a negative control. Two biological replicates are shown as points, and their average is the height of the bar. RPM = reads per million sequenced small RNAs