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Fig. 3 | Plant Methods

Fig. 3

From: A method to experimentally clamp leaf water content to defined values to assess its effects on apoplastic pH

Fig. 3

Measurement of leaf water content (LWC) and vapour pressure deficit (VPD) during an experiment using first (0–120 min) feedback-control of air humidity to perform a step change in air humidity and (120–280 min) feedback control of LWC to perform step changes of LWC. In the first part, leaf water content fluctuates dynamically as a result of changing leaf water balance. In the second part, LWC is fixed, and VPD is varied by feedback control as required to clamp LWC at constant set-points

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