Fig. 2
From: Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)
RT-PCR analysis of construct presence and integrity in N. benthamiana and P. virgatum. RT-PCR assays of systemic N. benthamiana (A) and P. virgatum (B) leaves and roots verifying the presence of the constructs FoMV:PDS, FoMV:ChlI or FoMV:ChlD as compared to wild type plants (no virus) and FoMV transfection only. Expected amplicon size is 314Â bp for FoMV, 410Â bp for FoMV:PDS, 424Â bp for FoMV:ChlI, and 369Â bp for FoMV:ChlD. Oligonucleotide sequences are listed in Additional file 1: Table S3