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Fig.1 | Plant Methods

Fig.1

From: Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)

Fig.1

Workflow for VIGS in switchgrass. Fragments of the target genes inserted into the foxtail mosaic vector (FoMV) system are first transfected into Nicotiana benthamiana leaves for multiplication of the virus. After PCR verification of successful transformation, leaf sap is extracted and used to mechanically rub-inoculate switchgrass leaves, followed by PCR-verification of transformants and gene expression analysis of target genes via RT-PCR

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