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Fig. 4 | Plant Methods

Fig. 4

From: MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein–protein interactions

Fig. 4

The sensitivity of the BiFC can be adjusted with different FP splits and promoters. A The promoter and FP fragment regions in the multigenic BiFC construct were varied to produce six different chloroplast targeted HSP21 pairs that were used for B BiFC assays in N. benthamiana mesophyll cells. NI, not inoculated. Scale, 10 µm; histogram levels shown at the bottom of each channel. Modules and plasmids are described in Additional file 2: Table S1 and full plasmid sequences are available in Additional file 4

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Plant Methods

ISSN: 1746-4811