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Fig. 3 | Plant Methods

Fig. 3

From: MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein–protein interactions

Fig. 3

N-terminal FP fusions function similarly to C-terminal fusions in BiFC experiments. A Chloroplast targeted HSP21 and HSP21/mCHERRY pairs with C-terminal and N-terminal FP fragment fusions were used for B BiFC assays in N. benthamiana mesophyll cells. NI, not inoculated; n, nYFP; c, cYFP; scale, 10 µm; histogram levels shown at the bottom of each channel. Modules and plasmids are described in Additional file 2: Table S1 and full plasmid sequences are available in Additional file 4

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Plant Methods

ISSN: 1746-4811