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Fig. 2 | Plant Methods

Fig. 2

From: MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein–protein interactions

Fig. 2

mCHERRY-nYFP and HSP21-cYFP do not produce a non-specific assembly signal in the chloroplast. A The POI region of the multigenic BiFC construction was varied to produce four different chloroplast targeted HSP21 pairs that were used for B BiFC assays in N. benthamiana mesophyll cells. NI, not inoculated; n, nYFP; c, cYFP; scale, 10 µm; histogram levels shown at the bottom of each channel. Modules and plasmids are described in Additional file 2: Table S1 and full plasmid sequences are available in Additional file 4

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Plant Methods

ISSN: 1746-4811